Yes.
In the old days, if you told Finder to copy very large numbers of files, it would sometimes choke with Error -43. Folder-to-folder "backups" with CCC didn't have that problem.
Note that if your source disk is formatted using APFS, and APFS has been doing "lazy" file copying, the backup might be significantly larger than the source. Backup software doesn't know about - or know how to copy - this APFS optimization. So even if two files share space "under the hood" on the source disk, they will each require space on the backup disk.